结晶紫-红霉素体系电荷转移分光光度法测定红霉素的研究

Spectrophotometric determination of erythromycin based on charge transfer reaction between erythromycin and methyl violet

目的:建立用结品紫电荷转移分光光度法测定红霉素的新方法。方法:以二次石英蒸馏水为溶剂,红霉素与结晶紫在60℃水浴中进行电荷转移反应生成1:1稳定的配合物,用紫外分光光度计于最大吸收波长593 nm 处测定其吸光度。结果:此法灵敏度高(ε=3.07×10~4 L·mol^(-1)·cm^(-1)),精密度较好(对25 mL 5.0×10^(-3)mg·mL^(-1)红霉素测定9次,RSD=2.9%)。红霉素的浓度在0～0.022 mg·mL^(-1)范围内服从 Beer 定律,方法的检出限为2.10μg·mL^(-1)。本法探讨了反应机理,其中红霉素作为电子给予体、结晶紫作为电子接受体发生了荷移反应。结论:本方法可靠,重现性好,可用于红霉素肠溶片和兽用红霉素含量的测定。

Objective ： A spectrophotometry method for the determination of erythromycin has been developed based on the charge transfer reaction between erythromycin and crystal violet in this paper. Method：The reaction was achieved with good results in water solution with 60 ℃ kept by water bath. Results：The apparent molar absorptivity of complex at 593 nm is 3.07×10^4L·mol^-1·cm^-1. The Beer＇s law was hold in the range of 0 -0. 022 mg·mL^-1 of erythromycin. Detection limit of this method was found to be 2. 10 μg·mL^-1. Conclusion：The method is reliable with good repeatability, this method can be applied to analysis of the erythromycin in entrie tablets and veterinary drugs.