HPLC-ELSD法测定盾叶薯蓣中黄姜素A的含量

HPLC-ELSD determination of Huangjiangsu-A in Dioscorea Zingiberensis

目的:采用 HPLC-ELSD 法测定盾叶薯蓣中(黄姜素 A 的含量。方法:药材粉末经60%乙醇回流提取,大孔树脂除杂富集后,以 HPLC-ELSD 法进行测定。色谱柱:Kromasil C_(18)(250 mm×4.6 mm,5μm),流动相:乙腈-水(35:65),流速:1.0mL·min^(-1),漂移管温度:100.8℃,气体流速:2.7 L·min^(-1)。结果:黄姜素 A 在0.0361～0.361 mg·mL^(-1)范围内有良好的线性关系,平均回收率为96.5%(n=6),RSD 为1.5%。结论:本方法简便,结果可靠,可用于盾叶薯蓣中黄姜素 A 的定量分析。

Objective ：To develop an HPLC - ELSD method for the determination of HJS - A in Dioscorea Zingiberensis. Method： After being extracted by refluxing with 60% EtOH, enriched through treated macroporous resin column, samples were achieved and separated by Kromasil C18 column （250 mm × 4. 6 mm, 5 μm） with acetonitrile - water （35：65 ） as mobile phase. The flow rate was 1.0 mL · min^-1. Results：HJS -A showed good linearity in the range of 0. 0361 -0. 361 mg · mL^-1, the average recovery was 96. 5 % （n = 6 ）, with RSD of 1.5 %. Conclusion： The method was simple, reliable and accurate, and can be used for the quality analysis of HJS - A in Dioscorea Zingiberensis rhizomes.