RP-HPLC法同时测定骨筋丸胶囊中士的宁和马钱子碱的含量

Simultaneous determination of strychnine and brucine in Gujinwan Capsules by RP-HPLC

目的:建立骨筋丸胶囊中士的宁和马钱子碱的反相高效液相分析法,为该药质量控制提供依据。方法:采用 Thermo Hy-persil C_(18) ODS 色谱柱(4.6 mm×250 mm,5μm);乙腈-甲醇-0.01 mol·L^(-1)KH_2PO_4-三乙胺(10:5:85:0.5,10%磷酸调 pH至2.5)为流动相;流速1 mL·min^(-1);检测波长254 nm。结果:士的宁在0.104～0.368μg(r=0.9999)、马钱子碱在0.072～0.252μg(r=0.9996)范围内呈良好的线性关系;士的宁回收率为99.22%(RSD=1.8%);马钱子碱回收率为100.01%(RSD=2.0%)。结论:本方法准确可靠,简便灵敏,重现性好,可用于骨筋丸胶囊中士的宁和马钱子碱的质量控制。

Objective：To establish a method for the determination of strychnine and brucine in Gujinwan Capsules by RP- HPLC for quality control of this preparation. Methods： Thermo Hypersil Cls ODS column （×250mm,5μm） and the solution of acetonitril- methanol- 0. 01 mol · L^-1 KH2PO4 -tfiethylamine （pH 2. 5 ） as mobile phase were used. The flow rate was 1 mL · min ^-1 with the detection wavelength at 254 nm. Results： Strychnine and brucine presented a good linear correlation in range of 0. 104 - 0. 368 μg（ r = 0. 9999 ） and 0. 072 - 0. 252 μg （r = 0. 9996）, respectively. The recoveries of them were 99. 22% （ RSD = 1.8% ） and 100. 01% （ RSD = 2.0% ）, respectively. Conclusions：This method is definite ,reliable and simple,and can be used for quality control of Gujinwan capsules.