河北道地药材知母HPLC-ELSD指纹图谱研究

Studies on HPLC-ELSD fingerprints of crude Rhizoma Anemarrhenae from Hebei province

目的:建立河北道地药材知母的 HPLC-ELSD 指纹图谱分析方法,并与不同产地知母药材指纹特征相比较,为科学评价与有效控制知母药材质量提供新方法。方法:采用 HPLC-ELSD 法测定了道地知母等19个不同来源的知母样品。色谱条件:Diamonsil^(TM) C_(18)(250 mm×4.6 mm,5 μm)色谱柱,乙腈(A)-水(B)为流动相,梯度洗脱:0～20 min,23%A;25 min,31%A;35 min,33%A;55 min,48%A;60 min,48%A;65 min,23%A。ELSD 检测器,流速1.0 mL·min^(-1),柱温30℃。结果:建立了知母 HPLC-ELSD 指纹图谱共有模式,并对不同产地知母药材进行了相似度比较。结论:方法简便、可靠,为全面有效控制知母药材的内在质量提供了依据。

Objective ：To establish a fingerprint analysis method for identification of Rhizoma Anemarrhenae of Hebei province,get the control chromatogram and compare the fingerprints of Rhizoma Anemarrhenae collected from different producing areas so as to establish a sensitive and specific method for controlling the quality of Rhizoma Anemarrhenae. Methods： The HPLC - ELSD fingerprints of Rhizoma Anemarrhenae from different places were obtained by Waters 1525 instruments. The HPLC separation was performed on a C18 analytical column（250 mm ×4. 6 mm,5 μm）gradient eluted with a mixture consisting of acetonitrile （A）and water（B） at a flow rate of 1.0 mL · min ^-1 with ELSD detector, using a linear gradient of 0 - 20 min ,23% A ;25 min ,31% A ;35 rain ,33% A ;55 min ,48% A ;60 min ,48% A ;65 min,23% A. The temperature of column was 30 ℃. Results： The mutual mode of HPLC - ELSD fingerprints was set up ,and the similarity degrees to the crude drugs of different producing areas were compared. Conclusions ：The method is simple and reliable and can be used as a quality control item for Rhizoma Anemarrhenae.