RP-HPLC法同时测定生脉注射液中人参皂苷Rb_1与五味子醇甲的含量

RP-HPLC determination of ginsenoside Rb_1 and schizandrol A in Shengmai injection

目的:建立生脉注射液中人参皂苷 Rb_1、五味子醇甲的含量测定方法。方法:采用反相高效液相色谱法,使用 KromasilC_(18)柱(250 mm×4.6 mm,5 μm),以乙腈-水梯度系统为流动相,流速1.1 mL·min^(-1),检测波长203 nm。结果:人参皂苷 Rb_1线性范围为75.0～750 μg·mL^(-1)(r=0.9999,n=6),平均回收率为98.5%(n=9);五味子醇甲线性范围为9.0～89.8 μg·mL^(-1)(r=0.9998,n=6),平均回收率为98.3%(n=9)。结论:本法操作简便、结果准确,为生脉注射液的质量评价提供了可靠的依据。

Objective：To establish a method for determination of contents of ginsenoside Rb1 and schizandrol A in Shengrnai injection. Method：An RP - HPLC method was developed. A Kromasil C18 column （250 mm × 4. 6 mm, 5 μm） was used, with the mobile phase of acetonitrile - water by gradient elution, the flow rate was 1.1 mL · min ^-1 and the UV absorbance was monitored at 203 nm. Results：The linear range of ginsenoside Rb1 was 75.0 -750μg · mL^- 1（r =0. 9999 ,n =6） ,the mean recovery was 98.5% （n =9） ;the linear range of sehizandrol A was 9.0 - 89. 8 μg · mL^-1 （r =0. 9998 ,n =6） ,the mean recovery was 98.3% （n =9）. Conclusion ：The method is proved to be simple and accurate, and can be used as a quantitative analytical method for the preparation.