反相高效液相色谱法测定三孢布拉霉菌超临界CO_2萃取物中番茄红素的含量

RP-HPLC determination of lycopene in supercritical carbon dioxide extract from Blakeslea trispora

目的:建立超临界 CO_2萃取-反相高效液相色谱法测定三孢布拉霉菌超临界 CO_2萃取物中番茄红素含量的方法。方法:采用美国 Alltech Alltima C_(18)(4.6 mm×250 mm,5 μm)色谱柱,以甲醇-四氢呋喃-水(70:26:4)为流动相,流速为1.5mL·min^(-1),检测波长为472 nm。结果:线性范围为0.1～0.5 μg,相关系数 r=0.998;精密度试验的 RSD(n=7)为1.7%,加样回收率为98.3%～102.1%。结论:本文采用反相高效液相色谱法初步建立了三孢布拉霉菌超临界 CO_2萃取产物中番茄红素的定量方法,该法分析准确、快速,灵敏度高,重现性好。

Objective：To establish an RP - HPLC method for determination of lycopene in Blakeslea trispora extracts by supercritical carbon dioxide extraction. Methods：The content of lycopene in Blakeslea trispora extracts was determined by reverse phase HPLC at 472 nm on an Alltima C18 （4.6 mm× 250 mm,5 μm）column with methanol -THF -water（70： 26： 4）as mobile phase and flow rate 1.5 mL · min^-1. Results：The linear range for the content of lycopene was between 0. 1 and 0.5 μg,r =0. 998 ,the recovery was 98.3% to 102.1% and RSD of precision test was 1. 7 % （ n = 7 ）. Conclusion： The method is simple, accurate and is suitable for the determination of lycopene.