盐酸西布曲明衍生光度法分析研究

Study and analysis on derivative spectrophotometry of sibutramine hydrochloride

目的:通过衍生反应,建立了用分光光度法测定盐酸西布曲明的新方法。方法:并对影响吸收强度的衍生体系、反应条件、产物稳定性等进行了研究。实验确定丙二酸和乙酸酐混合试剂与盐酸西布曲明在90℃的水浴中加热20 min,溶液呈深棕色,所得产物在370和328 nm 波长处有吸收峰,其中370 nm 处吸收最大。结果:在370 nm 波长处测得吸光度与盐酸西布曲明的浓度在0.15～12.5 μg·mL^(-1)范围内呈线性关系,遵守比尔定律,表观摩尔吸收系数为2.098×10~5 L·mol^(-1)·cm^(-1),检出限49 ng·mL^(-1),回收率为96.1%～102.6%。结论:本法用于曲美胶囊中盐酸西布曲明的测定,结果满意。

Objective：Based on derivative reaction, a sensitive derivative spectrophotometry method was developed for determination of sibutramine hydrochloride. Methods： The derivative system, reaction conditions, stability of the products were studied in detailed. The MAA system and sibutramine hydrochloride was simple to reaction was completed for 20 min at 90 ℃, got the deep brown liquid, the product had highly absorpti prepare and on at 370 nm and 328 nm, respectively, the maximum absorption wavelength of reaction product was located at 370 nm. Resuits ：The concentration of sibutramine hydrochloride in the range of 0. 15 -12. 5 μg · mL^-1 obeyed Beer＇ law, the apparent molar absorptivity was 2. 098 ×105 L · mol^- 1 . cm^-1 the detection limit was 49 ng · mL^-1the recovery was 96. 1% - 102. 6%. Conclusions： The method is applied to the determination of sibutramine hydrochloride in Qumei capsules, with satisfactory results.