当归多糖对小鼠外周血造血干细胞动员作用的研究

Mobilization of angelica polysaccharides on peripheral blood hematopoietic stem cell in mice

目的探讨当归多糖(APS)对小鼠外周血造血干细胞的动员作用。方法采用外周血白细胞(WBC)计数、淋巴细胞(LC)计数、免疫细胞化学、流式细胞术、造血祖细胞体外培养等实验技术观察APS的动员作用。结果APS动员后小鼠外周血的WBC、LC数明显高于生理盐水(NS)组(P<0.05);APS动员后外周血中CD34+细胞百分率明显高于NS组(P<0.05);APS组CFU-Mix产率显著高于NS组(P<0.01);并且APS和重组人粒细胞集落刺激因子(rhG-CSF)联合动员后的各项指标均高于单用APS或单用rhG-CSF组。结论APS对小鼠外周血造血干细胞有一定的动员作用,并且APS和rhG-CSF联合动员的效果好于单用APS或单用rhG-CSF的动员效果。

Objective To study angelica polysaccharides （APS） for mobilizing peripheral blood hematopoietic stem cell in BALB/c mice. Methods Techniques of peripheral white blood cell （WBC） and lymphocyte （LC） count, immunocytochemistry, flow cytometry, and hematopoietic cell culture in vitro were used to observe the APS mobilization. Results The numbers of WBC and LC in APS group were markedly higher than that in NS group （P〈0.05）; the percentage of CD34^＋ cells in peripheral blood in APS group was markedly higher than that in NS group （P〈0. 05） ; the yields of CFU-Mix in APS group was significantly more than that in NS group （P〈0.01）; the combined usage of APS and rhG-CSF enhanced more effects （P〈0. 05） than that in either single use of APS or rhG-CSF group. Conclusion The results indicate that APS could mobilize haernatopoietic stem cells into peripheral blood and the effect of APS in combination with rhG-CSF could be better than single use of APS or rhG-CSF.