不同品系黄连产量和质量的研究

Yield and quality of Coptis chinensis from different varieties

目的考察和比较湖北黄连GAP基地选育的3个不同品系黄连(大花叶、无光叶、小花叶)的产量和质量。方法采用田间随机区组设计,连续3年采集3个品系黄连根茎样品,分别以烘干法测定其质量,用紫外分光光度法和高压液相色谱法分别测定其总生物碱和盐酸小檗碱的量。结果在考察的3个品系药材样本中,各品系三年生黄连产量没有显著差异(P>0.05),在四年生和五年生黄连中,大花叶和无光叶品系产量均明显高于小花叶品系(P<0.05);在三年生、四年生、五年生黄连中,各品系总生物碱和盐酸小檗碱的量无明显差异(P>0.05)。结论大花叶和无光叶品系的黄连产量高、质量合格,可作为黄连规范化种植的种子基原。

Objective To study and compare the yield and quality of Coptis chinensis from three varieties （Dahuaye, Wuguangye, and Xiaohuaye） that bred in GAP cultivation habitat in Fubaoshan, Hubei Province. Methods Three samples were collected every year in the field, which were stochastically designed in past three-years and weighted by roasting for dryness. The weight and the contents of total alkaloids and berberine were determined by UV and HPLC, respectively. Results There was no significant difference among the three varieties on the yield of three-year-old samples （P〉0.05）, but the yields of four year-old and five-year old Dahuaye and Wuguangye samples were significantly higher than that of Xiaohuaye sample （P〈0. 05）, there were no significant differences among the three varieties on the contents of total alkaloids and berberine （P 〉 0.05）. Conclusion The varieties of Dahuaye and Wuguangye are excellent in higher yield and better quality, and both of them can be used as origin seeds in the GAP plantation of C. chinensis.