纳他霉素产生菌基因组重排育种

Natamycin-producing strain breeding by genome shuffling

Streptomyces gilvosporeus SG-1原生质体经紫外线诱变并筛选链霉素抗性菌株,获得纳他霉素高产菌株。在上述高产突变株中选择4株作为亲本进行基因组重排育种,筛选得到了高产重排菌株,其中一株重排菌株S.gilvosporeus GS-74的纳他霉素产量为3574mg/L,为产量最高的亲本菌株的153%,比原始出发菌株SG-1提高1.17倍。关键词:纳他霉素;褐黄孢链霉菌;

After isolating the protoplasts of Streptomyces gilvosporeus SG-1, they were mutated with UV irradiation, the streptomycin resistance mutants were screened, and the high natamycin producing strains were obtained. Among them, 4 strains with high natamycin production were selected as parent strains for genome shuffling. Consequently a high natamycin yeilding strain, S. gilvosporeus GS-74, was obtained, whose natamyein productiion reached 3574mg/L that was 153% of the parents strains and higher by 1.17 times than that of the starting strain.