高效液相色谱法测定普卢利沙星的含量

Content determination of prulixacin by HPLC

目的建立普卢利沙星的含量测定方法。方法采用高效液相色谱法；Alltima C18柱（150mm×4.6mm，5μm）；乙腈：0．005mol／L磷酸二氢钠溶液（取0．005mol／L磷酸二氢钠溶液100ml，加三乙胺0．2ml，用磷酸调节pH值至6.0）（40：60）为动相；流速1．0ml／min，检测波长278nm。结果普卢利沙星在12．8～204．1μg／ml浓度范围内呈现良好的线性关系，r=0.9999.结论该方法快速、简捷、准确都高，适用于普卢利沙星的含量测定。

Objective A high performance liquid chromatographic method for the content determination of prulixacin was developed. Method The HPLC was performed on a column of Alltima C18 （150mm × 4.6mm, 5μm）, using the mobile phase of 0.005mol/L sodium dihydrogen phosphate （added with 0. 2ml triethylamine and adjusted to pH 6.0 by phosphoric acid） ： acetonitrile （60 ： 40） at the detection wavelength of 278nm with the flow rate of 1.0ml/min. Results The calibration curve was linear in the range of 12.8～204.1μg/ml （r=0. 9999） for prulixacin. Conclusion The method was simple, quick and accurate for content determination of prulixacin.