牛病毒性腹泻/黏膜病病毒河北分离株的生物学特性

Biological Characteristics of Bovine Viral Diarrhoea-Mucosal Disease Virus HB Strain

【目的】了解牛病毒性腹泻病毒河北分离株HB株的特性,阐明牛病毒性腹泻/黏膜病致病机理,更好的控制BVDV感染、流行提供理论依据特进行此项试验;【方法】用理化学及生物学方法对BVDV河北分离毒株HB的特性进行检测,与BVDV准毒株OregonC24V株进行比较,分析了其理化性及生物学特性。电镜负染检查可见直径为40￣60nm有突起的圆形或近圆形的病毒颗粒,病毒在蔗糖中的浮密度为1.13￣1.14g/cm3。与牛病毒性腹泻-粘膜病病毒颗粒相似。毒力测定TCID50为10-4.5。理化学研究表明,该病毒对氯仿、乙醚、胰酶敏感;不耐酸、对碱具有较强的耐受性。牛病毒性腹泻/黏膜病病毒对56℃30min较敏感,56℃70min可使其完全灭活。血凝试验表明,该病毒对鸡、兔、猪和绵羊的红细胞均无血凝性;5-碘脱氧尿核苷(5,-IUDR)不能抑制病毒的增殖,核酸分型试验表明该病毒株基因组为RNA;病毒纯化后经SDS—PAGE电泳出现了5条主要结构蛋白带,与BVDV国际标准毒株OregonC24V株结果一致;【结论】河北分离株HB株为牛病毒性腹泻病毒。

[OBJECTIVE] Bovine Viral Diarrhoea-Mucosal Disease Virus HB strain was a field strain isolated from a dairy cows flock in HeBei province. The study were progressed in order to realize characters of HB strain, provide theory base for illuminating pathogeny and control infection and prevalence of BVDV. [METHOD] The biological characteristics of the virus HB strain were determined by Physicochemical and biological analysis methods. [RESULTS] The results indicated that the virus could be seen with typical MDV virions. By negative staining electron microscope, virions were roughly round and approximately 40-60 nm in diameter with peplomers. Density is 1.13-1.14g/cm^3. The hemagglutination reaction test proved that this virus had no any reaction to erythrocyte of chook, rabbit, pig and sheep. The result of TCID50 is 10^-4.5. Physicochemical assays showed that this virus was sensitive to chloroform and ether and trypsin. In acid alkali tolerance assay, the virus was sensitive to pH3.0 and resistant to pH9.0. The virus was sensitive to 56℃ for 30 minute and was inaetivited by heat to 56℃for 30 minute. It was similar to tandard strain in haemagglutinating activities morphological feature, density, the chemical and physical characteristics and Type of nucleic acid assay indicated that virions multiply could not be inhibited by 5,-IUDR. The virus genome is RNA. Five stripes of special virus structural protein were obtained by SDS-PAGE. [CONCLUSION] Virus HB Strain was a bovine viral diarrhoea-Mucosal disease virus strains.