复方杜仲对肉鸡免疫器官发育及ND-HI效价的影响

Effects of Compound Eucommia Ulmoides on Development of Immune Organs and ND-HI valence of Broilers

[目的]观察不同浓度复方杜仲对肉鸡免疫器官发育及新城疫血凝抑制(ND-HI)效价的影响。[方法]1日龄AA肉鸡400只随机均分4组。Ⅰ、Ⅱ、Ⅲ、Ⅳ组分别在饮水添加0g/L、1.25g/L、2.5g/L和5g/L的复方杜仲。每周末各组随机抽样8只称重、心脏采血、屠宰取胸腺、脾脏和法氏囊称重。[结果]1至6周胸腺指数、法氏囊指数各组间无差异。14日龄脾指数Ⅱ组高于Ⅰ组(P<0.05);21日龄Ⅱ组高于其他组(P<0.05),其中Ⅱ、Ⅲ组间差异极显著(P﹤0.01);42日龄复方杜仲Ⅱ、Ⅲ组脾指数均低于Ⅰ组(P<0.05),Ⅳ组极显著低于Ⅰ组(P<0.01)。21日龄Ⅲ、Ⅳ组ND-HI抗体水平高于Ⅰ组(P<0.05);28日龄达到最高水平,Ⅱ、Ⅲ组高于Ⅰ组(P﹤0.05),Ⅳ组极显著高于Ⅰ组(P﹤0.01)。[结论]复方杜仲水煎液对前3周肉鸡脾指数有一定的影响,且以低剂量组效果最佳。对ND-HI效价有增强作用,呈现量效递增关系。

[Objective] To observe the effects of Compound E. ulmoides with different concentration on the development of immune organs of broilers and ND-HI valence. [Methods] 400 AA broilers were divided into four groups at random. Group Ⅰ, Ⅱ, Ⅲ and Ⅳ were fed on Compound E. ulmoides at 0, 1.25, 2.5 and 5g/L. At each weekend, eight AA broilers in each group were weighed, took blood and slaughtered randomly. Then thymus, spleen, bursa of Fabriocius were collected and weighed in order to observe its effect on thymus index, spleen index, bursa of Fabriocius index of broilers. [Results] Thymus index and bursa of Fabriocius index had no differences with groups at the first six weeks. The Spleen index of broilers in groups Ⅱ was significantly higher than that in groups Ⅰ （P 〈0.05） at fourteen days old and obviously higher than any other group at twenty-one days old （P 〈0.05）. Among which group Ⅱ and Ⅲ had highly significant differences （P 〈0.01）. At forty-two-day old, the spleen index of groups Ⅱ and Ⅲ were all significantly lower than that in groups Ⅰ （P 〈0.05） while group Ⅳ was extremely lower than that in Group Ⅰ （P 〈0.01）; ND-HI antibody level of broilers in groups Ⅲ and Ⅳ were significantly higher than that in groups Ⅰ （P 〈0.05） at twenty-one days old. They reached the highest level at twenty-eight days old, ND-HI antibody level in groups Ⅱ and Ⅲ were significant higher than that in groups Ⅰ （P 〈 0.05） and group Ⅳ was higher than that in groups Ⅰ highly significantly （P 〈 0.01）. [Conclusion] The Compound E. ulmoides Aqueous Extract of different concentration had certain effect on the spleen index of broilers at the first three weeks, and the group with lowest concentration had the best effect. The Compound E. ulmoides Aqueous Extract could increase the effect of ND-HI valence. They presented a kind of relationship that the more the compound E. ulmoides Aqueous Extracts were given; the stronger the effect of the ND-HI valence appeared.