摘要
研究槲皮素对凝血酶诱导的血小板聚集和胞浆游离钙浓度的影响及钙对槲皮素的血小板聚集抑制效应的作用,本实验应用荧光钙离子指示剂观察槲皮素对血小板胞浆游离钙的影响。表明,槲皮素明显抑制凝血酶诱导的血小板聚集和游离钙的升高。IC50和95％可信区间分别为146.2(92.4～231.3)和78.5(49.5～124.4)μmol·L^(-1),槲皮素对凝血酶诱导的钙释放无影响,抑制钙内流是槲皮素抑制血小板聚集和〔ca^(2+)〕i升高的机制。
Aim:To study the effects of aue on the intraplatelet free catcium concentration and the effects of catcium on the inhibition of platelet aggregation by aue. methods : using auin - 2 fluorescence technique. Result :Que inhibited the platelet aggregation and the rise of [Cca2+]iinduced by thrornbin in platelets. The values of IC50 and 95% confidence interval were 146. 2(92. 4-124. 4)μmol/L,respectively. The inhibitory effects of Que o platelet aggregation induced by thrombin were reduced by adding catcium to the medium,and Que had no effect on thrombin -induced internal ca2+release from dense tubular system, conclusion : The inhibitory effects of que on aggregation and the rise of [ca2+]iin platelets was mainly due to an inhibition of ca2+influx
