氢醌对L-02肝细胞内POLH基因mRNA表达的影响

Effects of hydroquinone on the expression of POLH mRNA in human hepatic cells

目的 观察不同浓度氢醌（HQ）对L-02肝细胞内POLH基因mRNA表达的影响，旨在探讨HQ对人体细胞毒性作用的分子机制。方法 应用四甲基偶氮唑盐比色法检测不同浓度（0、5、10、20、40、80、160和320μmol／L）HQ作用24h后对L-02肝细胞存活率的影响；应用带有SYBR GreenⅠ的实时荧光定量聚合酶链反应技术研究不同浓度（0、5、10、20、40、80和160μmol／L）HQ对L-02肝细胞内POLH基因mRNA表达的影响。结果 在0—80μmol/L的范围内。HQ对L-02肝细胞的存活率没有明显的影响（P〉0．05），当染毒剂量超过160μmol／L时，其存活率则明显地下降（P〈0．01）；在HQ染毒剂量低于80μmol／L的范围内，POLH基因mRNA表达有随着剂量的增加而增加的趋势，当HQ的剂量达到160μmol／L时，POLH基因mRNA的表达则有所降低，但仍高于对照组。结论 HQ可以诱导L-02肝细胞内POLH基因在mRNA水平上的表达改变。

Objective To explore the effects of hydroquinone （HQ） on the expression of POLH mRNA in human L-02 hepatic cells, Methods MTT assay was conducted to detect the effects of HQ at various concentrations（0,5,10,20,40,80,160 and 320 μmol/L） on the survival rate of L-02 hepatic cells; real-time quantitative PCR with SYBR Green I technology was taken to measure the effects of HQ on the expression of POLH mRNA in L-02 hepatic cells exposed to HQ at various concentrations（0,5,10,20,40,80 and 160 μmol/L）. Results MTT assay showed that HQ at the concentrations from 0 to 80 μmol/L had little effects on survival rate of L-02（P 〉0. 05） ; whereas the survival rates in both groups of 160 and 320 μmol/L were significantly higher than that in the control （P 〈0.01 ） after treated with HQ for 24 hours. It was found that HQ at low concentrations （ 〈 80 μmol/L） could induce increased expression of POLH mRNA which was consistent with the increase of HQ concen- trations; the expression of POLH mRNA decreased once the concentrations of HQ exceeded 160 μmol/L when compared with that of the group 80 μmol/L,but it was higher than that of the control. Conclusion This study suggests that HQ might induce changes of the expression of POLH mRNA in hepatic cells.