摘要
为研究抗VacA+CagA+幽门螺杆菌(Hp)IgY的抗感染作用,以VacA+CagA+Hp为抗原免疫蛋鸡,聚乙二醇法和水稀释法从鸡卵黄中提取抗-VacA+CagA+Hp-IgY,酶联免疫吸附实验(ELISA)测定IgY抗体效价。建立胃腔感染VacA+CagAHp的昆明系小鼠模型,观察抗-VacA+CagA+Hp-IgY对小鼠胃腔感染VacA+CagA+Hp的防治效果。ELISA法测定IgY效价均为1∶20,480;抗-VacA+CagA+Hp-IgY防治小鼠胃腔感染VacA+CagAHp效果较理想,IgY高、中剂量组效果优于阳性对照组(P<0.05);低剂量组效果等同于阳性对照组(P>0.05)。抗-VacA+CagA+Hp-IgY较好的体内抗感染作用,提示该IgY有望成为较理想的治疗VacA+CagA+Hp感染的生物制剂。
To study effect of anti- VacA^＋ CagA^＋ Helicobacter pylori （Hp） -IgY against VacA^＋ CagA^＋ Hp, the titer of IgY extracted by PEG method and water dilution method from egg yolks of hens immunized with VacA^＋ CagA^＋ Hp was determined by enzyme linked immunosorbent assay （ ELISA ） . The Kunming mice model infected by VacA^＋ CagA^＋ Hp were created and applied to observe the prevention and treatment effects of anti- VacA^＋ CagA^＋ Hp-IgY against VacA^＋ CagA^＋ Hp infection in stomach of modal mice. The titer of anti- VacA^＋ CagA^＋ Hp-IgY is 1 ： 20, 480 by ELISA. The result in vivo showed that the IgY had good effects in protecting Kunming mice against VacA^＋ CagA^＋ Hp. The effects of high dose and medium dose IgY are better than that of the positive control （ P 〈 0. 05 ） . The effect of low dose IgY is nearly equal with that of positive control （ P 〉 0. 05 ） . The good effect of anti-VacA^＋ CagA^＋ Hp-IgY against VaeA^＋ CagA^＋ Hp in vivo indicates that it could be a hopeful orally biological preparation against VacA^＋ CagA^＋ Hp.
出处
《微生物学通报》
CAS
CSCD
北大核心
2006年第6期17-21,共5页
Microbiology China
基金
江西省卫生厅资助课题(No.20042016)
