显色培养基在几种食源性致病菌快速检测中的应用

Application of Chromogenic Media in some Food-borne Pathogenic Bacterial Rapid Detection

用显色培养基鉴定微生物是一种新的微生物快速检测技术,该技术以生化反应为基础,通过在培养基中加入细菌特异性酶的显色底物直接根据菌落颜色对菌种作出鉴定。常见食源性致病菌检测中,李斯特菌显色培养基(BCMTMListeriamonocytogenes,Rapid’LMONOagar,CHROMagarTMListeria)、大肠杆菌显色培养基(CHROMagarTMEcoli)、沙门氏菌显色培养基(Rambachagar)、金黄色葡萄球菌显色培养基(CHROMagarStaphylococcusaureus)等已被广泛应用于食品、医药和环境监测等领域,极大提高了微生物检测的效率。但是,显色培养基也存在一些假阳(阴)性等问题,其设计尚待优化。

Microbial detection by chromogcnic media is a new kind of microbial rapid detection technology, based on biochemical reaction, which may identify the bacteria according to the colony color through adding the bacterial special enzyme substrates to selective media. In food-borne pathogenic bacterial detection, Listeria chromogenic agar （BCMTM Listeria monocytogenes, Rapid＇ LMONO agar, CHROMagar^TM Listeria）, CHROMagar^TM Ecoli, Rambach agar and CHROMagar Staphylococcus aureus were widely used in many fields such as food, medicine and environmental inspection. That improved the detection efficiency greatly. However, there are some problems like false positive or false negative to be perfected in chromogenic media now.