鼠窒息后缺氧诱导因子1-α在心、肺中的表达

Expression of HIF1-α on Myocardium and Lung in Rats Model of Asphyxia Death

目的研究大鼠机械性窒息死亡后缺氧诱导因子1-α(HIF1-α)在心、肺中的表达变化,探讨其作为窒息死亡诊断指标的可行性。方法制作大鼠缢死模型,分别于窒息死后及正常断颈处死后0、2、6、24h时间段取材,用免疫组织化学、RT-PCR方法测定缢死后HIF1-α在心肌和肺组织中的分布和表达变化。结果HIF1-α在窒息组的心肌和肺组织中表达,窒息组和对照组在各时间段表现出明显差异,对照组仅在死后2、6、24h阳性表达。窒息死亡组可见核阳性表达,而对照组未见核阳性表达。半定量RT-PCR结果显示HIF1-α在0h时各组未见升高,但在2、6、24h窒息组则较对照组升高。结论心和肺组织HIF1-α表达核阳性细胞的出现,可以作为窒息死亡的特征性表现。

Objective To investigate the Methods The rats model of asphyxia expression of HIF1-α in heart and lung tissue died from asphyxia. death was constructed by hanging, different asphyxia groups and control group sets were made according the postmortem time （0,2,6,24 h）, immunohistochemistry and halfquantitative RT-PCR methods were used to investigate expression of HIF1-α and mRNA changes on heart and lung tissue. Results The positive staining of HIF1-α could be observed in the myocardium and lung tissue. Significant differences were found between the groups of asphyxia and their corresponding control group. HIF1-α expression was found in all the asphyxia groups while it was only expressed in the control groups of 2 h, 6 h and 24 h. Nucleic positive staining could be detected in all the asphyxia groups but none was found in the control groups. RT-PCR showed that the expression of mRNA between 0 h asphyxia group and Oh control group were equal in both cardic muscle and lung, but elevated expression in groups of 2,6,24h compared to their control groups. Conclusion The nuclear positive staining of HIF1- α in heart and lung can be a special character of suffocation death.