低氧诱导因子-1α在大鼠心肌梗死中的表达及其意义

Expression of hypoxia inducible factor-1α in rats with acute myocardial infarction

目的：观察低氧诱导因子-1α（HIF1α）在梗死心肌中mRNA表达水平的变化。方法：结扎SD大鼠冠状动脉左前降支制备心肌梗死模型。36只大鼠随机分为对照组（6只）和模型组（30只），模型组于冠状动脉结扎后24h、48～72h和1周时分别取左心室心肌梗死区及非梗死区组织．用半定量逆转录-聚合酶链反应（RT—PCR）检测HIF—1α mRNA的表达。结果：模型组不同时间点HIF-1α在梗死区心肌中mRNA表达水平均明显升高，且与非梗死区心肌比较差异均有显著性（P均〈0．01），在缺血l周内持续在高水平，模型组非梗死区域心肌组织与对照组HIF-1α mRNA水平比较差异无显著性（P均〉0．05）。结论：HIF1α mRNA在心肌梗死区的高表达是心肌对缺血和缺氧导致心肌坏死的一种分子水平的反应。

Objective： To explore the mRNA level expression of hypoxia inducible factor -1α （HIF-1α） in rats with acute myocardial infarction （AMI）. Methods： Myocardial infarction model was replicated by ligating left anterior descending branch of coronary artery in SD rats. Thirty-six rats were randomly divided into control group （n=6） and model group （n=30）. Semi-quantity reverse transcriptional polymerase chain reaction （RT -PCR） was used to test the mRNA level expression of HIF -1α in heart tissue of infarction and non-infarction areas at 24 hours, 48 - 72 hours and 1 week after ligation. The SPSS 11.5 software was applied to analyze the data： analysis of variance, paired t test and two samples t test for levels of HIF -1α mRNA. Results, The expression of HIF -1α mRNA was elevated in infarction area compared with non-infarction area in model group at different time points, and differences were obviously significant （all P〈0.01） and remained high level in 1 week after ischemia. The HIF -1α mRNA expression level had no differences in different stages in non infarction area in model group compared with that of control group （all P〉0.05）. Conclusion： The high level of HIF -1α mRNA expression happens at early AMI in infarction area. It is an early molecular marker of hypoxia and ischemia induced cardiac muscle apoptosis in myocardia infarction.