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Fibrillin操纵子在甘蓝型油菜和拟南芥中的瞬时表达 被引量：4

Transient Expression of Fibrillin Promoter in Protoplasts of Arabidopsis thaliana and Brassica napus
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摘要 将Fibrillin操纵子驱动的荧光素酶报告基因表达载体导入拟南芥原生质体中,研究基因表达调控,证明脱落酸(ABA)诱导报告基因的表达.同时将相同的质粒转化到甘蓝型油菜蜀杂9号原生质体中,获得与拟南芥中类似的表达结果.实验结果说明Fibrillin操纵子在拟南芥和油菜原生质体中均对ABA的诱导敏感. Arabidopsis fibrillin promoter was constructed in pBI221, and the plasmid was transferred into protoplasts of Arabidopsisor Brassicavia PEG-mediated method, showing a transient expression. After adding 30μmol/L ABA, it was found that the expression was upregulated, indicating fibrillin is sensitive to ABA in both Arabidopsis thaliana and Brassica napus.
作者 滕蕾 黄瑾 赵竹露 李旭锋 杨毅
机构地区 四川大学生命科学学院生物资源与生态环境教育部重点实验室
出处 《四川大学学报（自然科学版）》 CAS CSCD 北大核心 2006年第6期1394-1398,共5页 Journal of Sichuan University(Natural Science Edition)
基金 国家自然科学基金资助(项目批准号:30470927)
关键词 Fibrillin启动子 荧光素酶 PEG介导的转化 瞬时表达 脱落酸 fibrillin promoter luciferase PEG-mediated transformation transient expression ABA
分类号 Q78 [生物学—分子生物学]
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参考文献17

  • 1Demere J, Romer S, Harlingue A, et al. Fibril assembly and carotenoid overaeeumulation in ehromoplasts: a model for supramolecular lipoprotein structure[J]. Plant Cell, 1994, 6:119.
  • 2Pruvot G, Cuine S, Peltier G,et al. Charaeterization of a novel drought-induced 34-kDa protein located in the thylakoids of Solanum tuberosumL[J]. plants Planta,1996, 198(3): 471.
  • 3Vishenevesky M, Ovadis M, Itzhakl H, et al. Molecular cloning of a carotenold-associated protein from Cucumls sativuscorollas, homologous genes involved in earotenold sequestration in chromoplasts[J]. Plant J, 1996, 10(6) :1111.
  • 4Hyun Uk Kim, Sherry S H Wu, Chandra Ratnayake, et al. Brassica rapahas three genes that encode proteins associated with different neutral lipids in plastids of specific tissues[J]. Plant Physiotogy, 2001, 126:330.
  • 5Takaya Moriguehi, Masayuki Kita, Tomoko Endo-Inagaki, et al. Characterization of a eDNA homologous to carotenoid-associated protein in citrus fruits[J]. Bioehlmica et Biophysica Acta, 1998, 1442:334.
  • 6Kessler F, Sehnell D, Blobel G, et al. Identification of proteins associated with plastoglobules isolated from pea (pisum sativum L. ) chloroplasts[J]. Planta, 1999, 208.107.
  • 7Manac'h Nathalie, Kuntz Marcel. Stress induction of a nuclear gene encoding for a plastid protein is mediated by photo-oxidative events[J]. Plant Physiol.Biochem, 1999, 37(11):859.
  • 8Gillet B, Beyly A, Peltier G, et al. Molecular characterization of CDSP 34, a chloroplastic protein induced by water deficit in Solanum tuberosum L,plants, and regulation of CDSP 34 expression by ABA and high illumination[J]. Plant J, 1998, 16(2) : 257.
  • 9Langenkamper G, Manac'h N, Broin M, et al. Accumulation of plastid lipid-associated proteins(fibrillin/CDSP34) upon oxidativestress, aging and biotic stress in Solanaceae and in response to drought in other species[J]. J. of Experimental Botany, 2001,52(360) : 1545.
  • 10Yang Y, Sulpice R, Himmelbach A, et al. Fribrillin accumulation is regulated by ABI2 and is involved in abscisic acid-mediated photoprotection[J]. PNAS, 2006, 103: 6061.

二级参考文献7

  • 1程振东，植物生理学通讯，1992年，28卷，161页
  • 2卫志明，Plant Sci，1990年，70卷，101页
  • 3刘春明，遗传，1989年，11卷，39页
  • 4Li L C，Plant Cell Rep，1982年，1卷，209页
  • 5许智宏，Plant Sci Lett，1982年，24卷，117页
  • 6Lu D Y，Z Pflanzenphysiol，1982年，107卷，59页
  • 7卫志明,许智宏.影响花椰菜下胚轴原生质体培养和植株再生的因素[J].植物生理学报（0257-4829),1990,16(4):394-400. 被引量：11

共引文献17

同被引文献54

引证文献4

二级引证文献8

四川大学学报（自然科学版）
2006年 第6期

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