贵州从江侗族、威宁彝族、荔波瑶族谷胱甘肽S-转移酶M1、T1和P1的基因多态性研究

Frequencies of GSTM1, GSTT1, and GSTPl polymorphisms in Dong, Yi and Yao Nationalities from Guizhou

目的:研究贵州从江侗族、威宁彝族、荔波瑶族的GSTs基因多态性。方法:在隔离自然人群中,采用多重等位基因特异聚合酶链反应方法分析GSTM1和GSTT1基因多态性,同时采用PCR-RFLP的方法和TaqMan-MGB探针基因分型方法分析GSTP1 (A1578G)基因多态性。结果:贵州从江侗族、威宁彝族、荔波瑶族的GSTM1和GSTT1纯合缺失基因型频率分别为59．6％- 71．2％、39．4％-72．5％。其GSTP1(A1578G)基因型频率分别为:野生型(AA)为63-3％-75％、杂合子(AG)为23．2％-35．8％、纯合突变型(GG)为0-1．9％。等位基因频率:A为81．2％-86．6％,G为13．4％-18．8％。结论:贵州从江侗族、威宁彝族、荔波瑶族的GSTM1纯合缺失基因型频率在民族间差异无统计学意义,GSTP1(A1578G)基因型频率和等位基因频率在民族间差异无统计学意义,且其等位基因频率均符合Hardy-Weinberg平衡,但其GSTT1纯合缺失基因型频率在民族间差异有统计学意义(P<0．05)。

Objective： To investigate the frequencies of GSTM1 and GSTT1 polymorphisms in Dong, Yi and Yao nationalities from Guizhou. Methods： In 321 volunters, who are population-based, GSTMI and GSTT1 polymorphisms were analyzed by a Multiplex-PCR procedure, whereas GSTP1 polymorphism was analyzed by PCR-RFLP method and TaqMan-MGB Probes genotyping method. Results： We found that null genotype of 59.6%～71.2% and 39.4%～72.5% for GSTM1 and GSTT1, respectively. The genotypic distribution of GSTP1 was 63.3%～75% wild types （AA）,23.2%～35.8% heterozygotes （AG）,0～1.9% homozygotes （GG）, whereas the aUelic frequencies were 81.2%～86.6% for file A allele, and 13.4%～18.8% for the G allele. Conclusion： There was a significant relationship between GSTT1 frequencies and populations.