Calpain10基因多态性与2型糖尿病的相关性研究

Study on the correlation between Calpain10 gene polymorphism and type 2 diabetes mellitus

目的:探讨钙蛋白酶10(Calpain10)基因第3内含子SNP19多态性在湖北汉族人群2型糖尿病发生发展中的作用。方法:采用同胞对(家系内对照)和随机病例-时照两种实验设计,结合聚合酶链反应-限制性片断长度多态性(PCR-RFLP)技术分析共403个样本的Calpain10基因第3内含子SNP19多态性,并测定身高、体重、腰围、臀围、血压和空腹血糖等生理指标。结果:两种实验设计中对照组与病例组的基因型和基因频率均无显著差异(p 0．05)。结论:Calpain10基因SNP19多态性在湖北汉族人群2型糖尿病的发生发展中可能不起主要作用。

Objective： To investigate the correlation between Calpain10 gene SNP19 polymorphism of intron 3 and type 2 diabetes mellitus in Hubei Han nationality. Methods： Both sib-pairs and random case-control designs were used. The genotype in 403 samples were examined using polymerase chain reaction （PCR） . Height, weight , blood pressure and fasting blood sugar were measured. Results： In two experiment designs, the genotype and gene frequency between cases and controls had no significant difference （p〉0.05）, Conclusion： The occurrence and development of type 2 diabetes mellitus may be little associated with the SNP19 polymorphism of Calpain10 gene in the population of Hubei Han nationality.