摘要
目的:探讨钙蛋白酶10(Calpain10)基因第3内含子SNP19多态性在湖北汉族人群2型糖尿病发生发展中的作用。方法:采用同胞对(家系内对照)和随机病例-时照两种实验设计,结合聚合酶链反应-限制性片断长度多态性(PCR-RFLP)技术分析共403个样本的Calpain10基因第3内含子SNP19多态性,并测定身高、体重、腰围、臀围、血压和空腹血糖等生理指标。结果:两种实验设计中对照组与病例组的基因型和基因频率均无显著差异(p 0.05)。结论:Calpain10基因SNP19多态性在湖北汉族人群2型糖尿病的发生发展中可能不起主要作用。
Objective: To investigate the correlation between Calpain10 gene SNP19 polymorphism of intron 3 and type 2 diabetes mellitus in Hubei Han nationality. Methods: Both sib-pairs and random case-control designs were used. The genotype in 403 samples were examined using polymerase chain reaction (PCR) . Height, weight , blood pressure and fasting blood sugar were measured. Results: In two experiment designs, the genotype and gene frequency between cases and controls had no significant difference (p〉0.05), Conclusion: The occurrence and development of type 2 diabetes mellitus may be little associated with the SNP19 polymorphism of Calpain10 gene in the population of Hubei Han nationality.
出处
《现代生物医学进展》
CAS
2006年第11期41-43,共3页
Progress in Modern Biomedicine
基金
国家自然科学基金资助项目(30340068)