气相色谱法测定麝香祛痛凝胶的4种有效成分的含量

Simultaneous Determination of four ingredients in Shexiang Qutong Gel by GC

目的:采用气相色谱法,程序升温方式同时测定麝香祛痛凝胶中主要成分麝香酮、樟脑、薄荷脑、冰片的含量。方法:采用玻璃柱3 m×3．2 mm,担体Chro2mosorb W 60-80目,涂布6％聚乙二醇(PEG)-20M,2％苯基(50％)甲基硅酮(OV-17)。从80℃到180℃程序升温;载气:高纯氮,流量:50ml·min-1;FID检测器;进样体积6μL。结果:试验表明,樟脑、薄荷脑、冰片、麝香酮分别在1-8μg,0．6-4．8μg,1-8μg,0．15-1．2μg范围内呈良好的线性关系,回归方程分别为Y=18 062 X-483(r=0．999 9),Y= 9 829 X+61(r=0．999 6),Y=21 006X+562(r=0．999 1),Y=286 986X+406(r=0．999 4)。平均回收率分别为99．78％,100．9％,98．81％,98．68(n=5)。结论:该法可靠简便,结果准确,可作为控制麝香祛痛凝胶质量的方法。

Objective： To determine Camphor, Mentholum , Bomeol and Muscone in Shexiang Qutong Gel by gas chro- matography.Methods： FID detector; pogrammed temperature from 80 ℃ to 180℃; cartier gas, 50ml·min^-1 N2. Results： The linear ranges were 1-8μg（Camphor）（Y=18 062 X-483, r=0.999 9）, 0.6～4.8μg（Mentholum）（Y=9 829X＋61, r=0.999 6）, 1 ～8μg（Borneol） （Y=21 006X＋562, r=0.999 1）, 0.15～1.2μg（Muscone）（Y=286 986X＋406, r=0.999 4） respectively. The average recovery rates were 99.78 % and 100.9 % and 98.81% and 98.68 respectively（n=5）. Conclusion： This method is simple,rapid and accurate, and it can be used as a method of quality control.