巯基棉分离富集-胶束增敏荧光分析法测定痕量汞

Determination of Trace Hg （ Ⅱ ） by Miceilar - sensitized Fluorescence Spectrophotometry with Separating and Enriching of Sulphydryl Cotton

实验发现，在pH7．8的KH2P04-NaOH缓冲介质中，十二烷基硫酸钠（SDS）可使Hg（Ⅱ）与吖啶橙（AO）反应后的荧光明显增强，据此建立了测定汞的荧光分析新方法。在最佳实验条件下其浓度线性范围为0—200μg／L，检出限为2，47×10^-3mg／L。新建方法联结巯基棉分离富集汞，用于环境水样中痕量汞的测定。相对标准偏差为2．1％-一4．7％，样品加标回收率为99．3％-101．2％，结果满意。

The experiments indicated that SDS micellar systems could enhance greatly the fluorescence signal of Hg （ Ⅱ ） - AO in pH 7.8KH2P04 - NaOH. A new method for the determination of trace Hg （ Ⅱ ） by rnicellar - sensitized fluorescence spectrophotometry was established. In the optimal analysis conditions, the linear range was 0 - 200g/ L.The determination limit was 2.47 x 10^-3 g/mL. When proposed method was used to determine Hg （ Ⅱ ） in water by Separating and Enriching Sulphydryl Cotton, satisfactory results were obtained , the relative standard deviation varied from 2.1% to 4.7% and the recoveries from 99.3% to 101.2%.