嘉陵江C市段水中有机污染物睾丸毒性分子机制初步研究

Mechanism of rat testis toxicity induced by organic extract from Jialing river water

目的探讨水中有机污染物对大鼠睾丸损伤的分子机制。方法采用XAD-2树脂浓缩水中有机污染物;大鼠经灌胃暴露于水中有机提取物,用TUNEL法检测细胞凋亡;免疫组化与免疫印迹方法检测水中有机提取物对fas,fasL,scf,c-k it蛋白表达的影响。结果高剂量组水中有机污染物可增加精子细胞凋亡率(P<0.05),精原细胞也观察到凋亡,但实验组与对照组之间没有统计学差异。高剂量组水中有机污染物可明显增加精原细胞fas,fasL和c-k it蛋白表达(P<0.05)。结论水中有机污染物对精原细胞的发育分化有影响,并可诱导精子细胞凋亡,有可能破坏精原细胞的生精过程,但详细机制还需进一步研究。

Objective To study the toxicity of organic extract from river water on the male reproductive system in rats. Methods Organic pollutants were extracted from river water with XAD-2 resin and the extract from 2, 16 and 80 L river water was administered daily into 30 male SD rats by gavage for consecutive 28 days. Corn oil served as the solvent control in another ten rats. After the last intake of water extract, all rats were killed, and the sections of testis were prepared according to routine procedure. The TUNEL assay for in situ detection of apoptosis and quantitation of apoptotic germ were performed on testicular sections. Immunohistochemistry and Western blotting were used to examine the changes of scf, fas, fasL and c-kit expressions in rat testis. Re- suits The percentage of apoptotic spermatid cells significantly increased in rats receiving organic extract from 80 L water daily, as compared with the control rats. The spermatocytes in rats receiving organic extract from 80 L water daily overexpressed fas, fasL and c-kit. Conclusion The organic pollutants from river water result in spermatid apoptosis and spermatogenesis arrest.