摘要
目的复制小鼠→大鼠异种心脏移植模型,探讨核转录因子κB(nuc lear factoκr-B,NFκ-B)P65蛋白在异种移植心肌中的表达及NFκ-B的DNA结合活性。方法动物随机数字法分成2组:A组(对照组),n=6;B组(移植组),n=6。采用免疫印迹杂交法(W estern b lot)检测对照组及移植组心肌组织NFκ-B P65表达,凝胶电泳迁移率实验检测NFκ-B DNA结合活性。结果移植组的NFκ-B P65蛋白表达和NFκ-B DNA结合活性均显著高于对照组(P<0.05)。结论NFκ-B的激活可能在大鼠异种移植心脏排斥反应中起着重要作用。
Objective To reproduce mouse to rat heart transplantation model and determine the expression and DNA binding activity of NF-κB P65 in xenograft heart. Methods Six NIH mice were used as heart donors and six Wistar rats as heart recipients. After the transplantation, the transplanted hearts without any drug intervention were prepared for experiment after cardiac arrest. The hearts from another six NIH mice served as norreal controls. The expression of NF-κB P65 protein and NF-κB P65 DNA binding activity in xenograft heart were determined by Western blotting and electrophoretie mobility shift assay (EMSA). Results The expression level of NF-κB P65 and NF-κB P65 DNA binding activity in transplanted hearts were higher than control hearts (P 〈0.05). Conclusion NF-κB may play an important role in mouse to rat heart transplantation.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2007年第1期51-52,共2页
Journal of Third Military Medical University
基金
重庆市卫生局科研课题(05-2-180)~~