三氧化二砷诱导人卵巢癌细胞凋亡对端粒酶活性的影响

Effect of human ovarian cancer cell apoptosis induced by arsenic trioxide on telomerase activity

目的探讨As2O3诱导细胞凋亡时对其端粒酶活性的影响及其作用机制。方法采用光镜、电镜,PCR-ELISA,RT-PCR等技术,检测细胞凋亡,端粒酶活性及其催化亚单位的表达。结果As2O3可诱导卵巢癌细胞凋亡,但存在作用浓度,细胞株类型差异性;端粒酶活性随药物作用时间和浓度的增加而明显减弱,同时伴随hTERT mRNA表达水平的下降。结论端粒酶活性和hTERT mRNA表达在As2O3诱导SKOV3、3AO细胞凋亡过程中共同发挥作用,两者有密切关系,是诱导细胞凋亡的分子机制之一。

Objective To investicate the effect of apoptosis of human ovarian cancer cell line SKOV3 and 3AO exposed to arsenic trioxide on telomerase activity and its mechanisms. Methods The human ovarian cancer cell line SKOV3 and 3OA were treated with arsenic trioxide of different concentration for 12, 24, 72 h. Cell morphology, PCR-ELISA, RT-PCR were adopted to detect the cell apoptosis and telomerase activity and the expression of human telomerase catalytic subunit （hTERT）. Results Arsenic trixide could induce apoptisis of ovarian cancer cell lines, but there exists difference in drug concentration, type of cell line. A dose and time-dependent decline of telomerase activity after SKOV3 and 3AO ceils exposed to arsenic trixide, meanwhile hTERT mRNA was down-regulated. Conclusion Telomerase activity and hTERT mRNA expression have close relationship and play an important role in arsenic trioxide inducing SKOV3 and 3AO ceils apoptosis.