环磷酰胺对人腺泡状横纹肌肉瘤细胞裸鼠移植瘤生长和形态结构的影响

Effects of cyclophosphamide on proliferation and morphology of human alveolar rhabdomyosarcoma cell xenografted tumor in nude mice

目的检测环磷酰胺(cyc lophospham ide,CTX)对人腺泡状横纹肌肉瘤细胞株PLA-802裸鼠移植瘤生长和形态结构的影响。方法16只裸鼠右腋皮下接种PLA-802移植瘤细胞悬液后分成空白对照组(5只)、生理盐水对照组(5只)和20 mg/kg CTX治疗组(6只)。接种后第7天开始,经腹腔注射给药,隔日1次,共注射10次。每2天测量1次肿瘤大小绘制移植瘤生长曲线。接种后第26天杀鼠摘取移植瘤称重,组织经制片染色后用光镜和电镜检查。结果①CTX治疗组移植瘤生长被明显抑制(P<0.05),抑瘤率85.55%。②治疗后瘤细胞疏松排列成腺泡状,由纤维间质分隔,血管稀少;瘤细胞椭圆形、网拍状或短梭形;核椭圆或类圆形,大小不一;核分裂相减少。电镜见瘤细胞质内有不等量肌(微)丝,甚至有横纹肌肌原纤维结构形成。结论CTX对人腺泡状横纹肌肉瘤PLA-802移植瘤生长具有一定程度的抑制作用,治疗后瘤组织形态结构改变。

Objective To investigate the effects of cyclophosphamide （CTX） on proliferation and morphology of human alveolar rhabdomyosarcoma cell-line PLA-802 xenografted tumor in nude mice, Methods Sixteen nude mice which had been implanted PLA-802 by subcutaneous injection into the right armpit were divided into control group （5 mice）, normal saline group （5 mice） and 20 mg/kg CTX group （6 mice ）. They were given CTX or normal saline intraperitoneally once every two days, starting on the 7^th day after tumor cell transplantation. Tumor volumes were measured every two days for drawing the growth curve. Animals were anesthetized on the 26^th day after tumor cell transplantation to get weight and specimens of the tumors. Specimens were stained of HE or EM, and then examined with light microscope and electron microscope. Results The proliferation of xenografted tumors was inhibited significantly （ P 〈 0.05 ）. The inhibition rate of the tumors was 85.55 %. The morphology of xenografted tumor was changed after CTX treatment. It appeared that neoplastic cells arranged in alveolar form, which were comparted by fibrous tissue and were of fewer blood vessels. Neo- plastic cells were oval, racket-shape or short fusiform, and nucleus were oval or round-like in various sizes. Karyokinesis was less. There were various amount of myofilament, even muscle myofibril in cytoplasm. Conclusion CTX can inhibit the proliferation and change the morphology of PLA-802 xenografted tumor.