摘要
目的探讨癌基因HER-2反义寡核苷酸联合放疗对乳腺癌细胞的影响。方法实验分组如下:HER-2反义寡脱氧核苷酸组、HER-2正义寡脱氧核苷酸组、叶酸对照组和空白对照组。用RT-PCR方法、MTT法及平板克隆形成实验检测乳腺癌细胞SK-Br-3受药物处理后,经60Coγ射线照射,HER-2表达水平、细胞存活分数和集落形成率的改变。结果经HER-2反义寡核苷酸-叶酸处理的细胞,对60Coγ射线的放射敏感性增加,细胞的HER-2癌基因表达水平降低,存活分数及集落形成率与正义寡核苷酸、叶酸和空白对照组比较明显降低(P<0.05),而正义寡核苷酸、叶酸与空白对照组相比无明显差异(P>0.05)。结论HER-2反义寡核苷酸抑制癌基因表达的作用增加乳腺癌细胞SK-Br-3对放疗的敏感性。
Objective To explore the effect of HER-2 oncogene antisense oligodeoxynucleotides on radiosensitivity of breast cancer cell line. Methods To set up HER-2 antisense oligodeoxynucleotides （ASODN） experimental group, sense oligodeoxynueleotides （SODN） group, folate acid （FA） group and ^60Co irradiation control group. The expression of HER 2 was detected by means of RT-PCR. Cel lular response to irradiation was evaluated by the colony forming test and MTT assay. Results HER-2 ASODN could suppress the expression of c-erbB2 which significantly increased the radiosensitivity of breast cancer cells （P〈0. 05）, while folate acid group and the SODN group did not increase the radiosensilivity of SK-Br-3 cell line. Conclusion HER 2 antisense oligodeoxynucleotides sensitized the breast cancer cell SK Br-3 to ionizing irradiation through decreasing the expression of HER-2.
出处
《贵州医药》
CAS
2006年第12期1059-1062,共4页
Guizhou Medical Journal
基金
贵州省优秀科技教育人才省长专项资金资助项目[编号:黔省专合字(2005)36号]
