摘要
目的探讨重症急性胰腺炎(severe acute pancreatitis,SAP)并发急性肺损伤(acutelung injure,ALI)之大鼠P-选择素浓度的变化及其机制。方法取20只大鼠,随机分为假手术组(A组)及胰腺炎组(B组),每组10只。胰管内逆行性注射3%牛磺胆酸钠的方法建立SAP并发ALI大鼠模型,24小时后检测血清淀粉酶、P-选择素和肺组织P-选择素浓度,并取胰腺和肺组织做病理形态学检查。结果制模后B组大鼠血清淀粉酶、P-选择素和肺组织P-选择素浓度较对照组显著升高(P<0.05);A组胸、腹腔内及胰腺、肺组织均无明显病理变化。B组腹水形成,腹腔内组织广泛分布有皂化斑,胰腺广泛水肿、出血、坏死,胸腔内有胸水形成,肺组织存在组织结构紊乱,灶性或片状肺不张,肺泡腔及间质出血等病理学改变,胰腺及肺组织均发现有大量的中性粒细胞浸润。结论SAP合并ALI时,血清P-选择素和肺组织P-选择素浓度均同时异常升高,提示它们在其发病机制中可能具有重要作用。
Objective To study of P selectin of rats with Severe Acute Pancreatitis with Lung Injury. Methods 20 healthy Wistar rats were randomly divided into two groups： normal group （A group） and SAP group （B group）. The model of severe acute pancreatitis was induced by retrograde injection of 3% sodium taurocholate into bilio-panereatie duct in group B. All rats were sacrificed, and blood was drawn and collected from portal vein after SAP had been produced fro 24 hours. The concentration of serum amylase, P-selectin and pulmonary tissue P-selectin were measured. Pathologic alteration of pancreas and lung were observed under microscope. Results The concentration of serum amylase, P-selectin and pulmonary tissue P-selectin were lower in normal rats than that in SAP rats （P〈-20. 05）. Conclusion P-selectin plays an important role in rats with severe acute pancreatitis with lung injury.
出处
《贵州医药》
CAS
2006年第12期1066-1068,共3页
Guizhou Medical Journal
基金
贵州省科技厅中医药现代化攻关项目[2003(13)]
