杂交鹅掌楸体胚系统的遗传稳定性研究

Studies on the Genetic Stability of Embryogenic Cellular Lines of Liriodendron Hybrids

以1999年建立的杂交鹅掌楸体细胞胚胎发生体系为研究材料,进行长期继代培养的胚性细胞系的增殖能力及再生植株遗传稳定性研究。结果表明:建立的体细胞胚胎再生体系经过3a甚至6a的继代培养,能够保持旺盛的体胚发生能力;染色体计数发现多数细胞系的再生植株遗传稳定性较好,为二倍体再生植株,仅在一个细胞无性系当中发现所有再生植株均为四倍体。

The research on the propagation ability and plant genetic stability was carried on with the long-term cultured embryogenic cell lines of Liriodendron hybrids which was established in 1999 as study material. The results showed that the embryogenic cell lines kept high frequency regeneration ability after three years, even six years of sub-culture. The genetic stability of the regenerated plantlets had been studied by chromosome numeration and it was found that these somaplants were diploid mostly, while only one of the somaclones regenerated plants with tetraploid karyotype.