杨树新梢萌发过程中贮藏蛋白质的动态变化

The Dynamic Changes of Vegetative Storage Proteins in Poplar During the Course of Sprouting

采用凝胶电泳技术(SDS-PAGE)对1年生杨树无性系797杨春季芽萌发阶段皮层、木质部和根系中的蛋白质含量及营养贮藏蛋白质组分进行了测定分析。结果表明:杨树体内(木质部、皮层、根部)蛋白质含量在芽萌发期间处于最低水平;木质部和皮层是杨树越冬期间贮藏蛋白质的主要场所;杨树新梢萌发期间贮藏蛋白质组分变化最明显的是36、32、181、6 kD等蛋白,其中36、32 kD蛋白在萌发前均可以清晰地辨认,叶子展开4周以后逐渐消失,而18、16 kD两种蛋白仅在发叶期间出现,之后完全消失,36、32 kD蛋白是杨树营养贮藏蛋白质的主要类型。

The contents of proteins and the compound of vegetative storage proteins in the bark, xylem and roots during the course of sprouting of the one-year-old poplar clone 797 have been measured and analyzed by SDS-PAGE. The results indicated that the content of proteins in the bark, xylem and root decreased obviously during the course of sprouting. The vegetative storage proteins mainly stored in the bark and xylem in winter, the contents in the root were relatively low at that time. The proteins such as 36 kD, 32 kD, 18 kD, 16 kD and etc. changed greatly during the course of the poplar shoots sprouting, of which the proteins 36 kD, 32 kD could be recognized clearly before sprouting and disappeared gradually 4 weeks after the leaves spreading, while the proteins 18 kD, 16 kD could be found only during the leaves growing and disappeared completely afterwards. The proteins 36 kD, 32 kD were probably the main types of vegetative storage proteins of poplar.