部分水解聚丙烯酰胺生物降解的初步研究

Study on Biodegradation of Partially Hydrolyzed Polyacrylamide

聚丙烯酰胺降解细菌G1能在一定浓度的聚丙烯酰胺溶液中生长繁殖,具有降解水解聚丙烯酰胺(HPAM)并降低其溶液黏度的能力。实验通过改变HPAM溶液浓度、pH和降解菌初始接种量、培养温度、培养时间、及连续活化次数等,探究G1菌对HPAM溶液的降解特性。实验结果表明:G1菌连续活化3次,接种量10%,在浓度10 g.L-1HPAM的溶液中,30℃恒温振荡培养10 d,可使溶液黏度损失率达到29.8%。

A strain G1 which degraded polyacrylamide was researched. It could grow in polyacrylamide solution, degrade hydrolyzed polyacrylamide（HPAM） and reduce the viscidity of solution. In this study, the effects of concentration of solution, pH, initial inoculated bacteria size, culturing temperature, culturing time and continuous activating times on the viscosity of HPAM were discussed. The experimental results show that after 3 times activation of the G, and 10% initial inoculated bacteria size the viscosity loss of 10 g ·L^-1 HPAM solution reach 29.8% in 10 days cultivation under 30 ℃.