恶性肿瘤患者化疗前后外周血免疫学指标的检测及其临床意义

ASSESSING IMMUNOLOGICAL CELL ACTIVITY IN MALIGNANT-TUMOR PATIENTS BEFORE AND AFTER CHEMOTHERAPY

目的观察恶性肿瘤患者化疗前后外周血免疫学指标的变化,并探讨其临床意义。方法应用流式细胞仪检测103例恶性肿瘤患者静脉化疗前、后外周血T淋巴细胞亚群(CD3+、CD4+、CD8+)、B淋巴细胞(CD19+)及NK细胞(CD3-/CD16+56+)的表达率。结果所有患者化疗后CD3+、CD4+、CD4+/CD8+表达高于化疗前(P<0.05),其中化疗有效组(30例),差异有显著性意义(P<0.01),化疗无效组(40例),差异无统计学意义(P>0.05);化疗前后CD19+的变化无统计学差异(P>0.05);化疗后NK细胞降低明显,有统计学差异(P<0.05)。结论有效的化疗能够改善恶性肿瘤患者的T淋巴细胞免疫功能。

Objective To explore the effects of chemotherapy on the immunity function of patients with malignant tumor and analyze its clinical significance. Methods Lymphocyte subgroups and natural killer cell in peripheral blood from 103 patients with malignant tumor were measured with flow cytometry （FCM）. Results In comparison with samples before chemothraphy, the expresssion of CD3^＋ , CD4 ^＋ , CD4 ^＋/CD8^＋ increased significantly （ P 〈 0.05） ; especially in the response group（30 cases）; the differences were more obvious（ P 〈 0.01 ） in the no-response group（40 cases）, the expression of CD3 ^＋ , CD4^＋ , CD4 ^＋/Cd8^＋ also increased, but without much difference （P〉0.05）, Both in the response and no-response groups, the expression of CD3^-/CD16＋56^＋ decreased after chemotheraphy（ P 〈 0, 05）, B cells had no obvious change before and after chemotheraphy（P 〉0,05）, Conclusions Proper chemotherapy can change the cell immune status and increase the activity of immunity function of patients with malignant tumor.