摘要
目的:观察缬沙坦对高浓度C反应蛋白(CRP)诱导的正常人外周血单核细胞合成白细胞介素-6(IL-6)的影响,探讨缬沙坦的抗炎作用。方法:采用密度梯度离心法分离人外周血单核细胞,应用酶联免疫吸附试验分别观察CRP刺激单核细胞产生IL-6的时间及剂量效应,其峰值与缬沙坦抑制剂组比较。结果:CRP刺激单核细胞IL-6合成呈时间依赖性和剂量依赖性,50 mg/L CRP诱导IL-6合成开始的时间是4 h,在24 h达高峰,其峰值是1 204±272 ng/L;仅高浓度缬沙坦(1×10-3mol/L)能抑制50 mg/LCRP诱导的单核细胞IL-6合成。结论:缬沙坦在体外能抑制CRP诱导单核细胞产生IL-6,但这一效果仅在体内并不能达到的高浓度时产生。
Objective:To evalulate the effect of valsartan on interleukin-6 production in human monocytes stimulated by 50 mg/L c-reactive protein(CRP),and assess the influence of valsartan on monocytes inflammatory reaction.Methods:Monocytes were isolated by Ficoll density gradient from blood of healthy volunteers.And ELISA was adopted to measure IL-6 production stimulated by 50 mg/L CRP at 2,4,8,16,24 hours and 50 mg/L CRP at 24 hours,the peak to be compared with that of the group valsartan(1×10^(-6) ~ 1×10^(-3) mol/L).Result:IL-6 could be produced after being stimulated with 50 mg/L CRP at 4 h.The effect of CRP was dose-dependent and time-dependent,reaching peak at 24 h.And the highest level of monocytes IL-6 was 1 204 ± 272 ng/L in CRP group.Valsartan(1×10^(-3) mol/L) inhibited IL-6 production induced by 50 mg/L CRP.Conclusion:Valsartan is able to inhibit IL-6 production induced by 50 mg/L CRP in vitro,but this effect isn,t shown in vivo.
基金
长江大学医学院科研基金资助(编号:200304)
