吖啶橙染色法在膀胱癌无创诊断中的应用

Application of Acridine Orange Method(AO-F)in the Non-traumatic Diagnosis of Bladder Cancer

[目的]运用吖啶橙染色荧光显微镜(acridineorenge,AO-F)检查法进行膀胱癌无创性诊断。[方法]采用AO-F检查法及巴氏染色分级(papanicolaousstaingradingsmethod,PG)检查法检测79例膀胱癌患者及80例非肿瘤患者的尿脱落细胞。[结果]两种染色法对诊断膀胱癌均有统计学意义,分层卡方检验,χMH2=173.461,P=0.000。巴氏染色法与吖啶橙荧光染色法比较,后者膀胱癌的检出率优于前者,χ2=19.606,P=0.000。巴氏染色法检出膀胱癌的敏感度为56.6%,特异度为100%。吖啶橙荧光染色法检出膀胱癌的敏感度为85.5%,特异度为100%。80例非膀胱癌患者两种尿脱落细胞检查的阳性检出率为0%。[结论]吖啶橙染色法比单从细胞形态上检测肿瘤细胞更具有重要的临床价值,可作为膀胱癌的辅助诊断方法。

[ Objective ] To apply the acridine orange fluorescence microscope method （AO-F） for non-traumatic diagnosis of bladder cancer. [ Methods ] The exfoliated urothelial cells of 79 bladder cancer patients and 80 non-tumorous patients were determined with the AO-F and Papanicolaous grading method （PG）. [ Results ] There was statistical significance to diagnose the bladder cancer with these two methods. （χMH^2=173.461, P=0.000. ） The positive rate of AO-F method was higher than that of the PG method. （χ^2=19.606, P=0.000 ）. The sensitivities of PC, and AO-F methods were 56.6% and 85.5% respectively. The speeifieities of PC, and AO-F methods were both 100%. [ Conclusion ] The clinical value of acridine orange method （ AO-F ） is higher than the cell morphologic method. It can become an auxiliary diagnosis method for bladder cancer.