益骨汤对去卵巢大鼠血清激素及成骨细胞增殖的影响

INFLUENCE OF "YI GU TANG" ON SERUM HORMONE AND OSTEOBLAST PROLIFERATION IN OVARIECTOMIZED RAT

为观察益骨汤对去卵巢大鼠骨钙素(BGP)、降钙素(CT)及成骨细胞增殖影响。取12周龄Wistar雌性大鼠40只,随机分为A组(模型组)、B组(治疗组)、C组(阳性对照组)、D组(正常对照组),每组10只,A、B、C组行完整双侧卵巢摘除术,D组行假手术,术后常规饲养12周,再分别予以生理盐水、益骨汤水提液、-αD3水溶液及生理盐水灌胃治疗12周,处死大鼠,取血清用放免法测定血清中BGP、CT水平;取右胫骨上端做病理切片,通过HE染色对成骨细胞进行观察比较。结果显示治疗组血清BGP、CT水平明显高于模型组,HE染色发现其成骨细胞数量明显增加。表明益骨汤能促进成骨细胞增殖,提高血清BGP、CT水平,抑制骨吸收,加快骨形成,具有治疗OP的作用。

In order to observe the effect of 'YI GU TANG'(YGT,a TCD decoction for osteoporosis) on BGP,CT and osteoblasts proliferation in ovariectomized rat,40 male Wistar rats aged 12 weeks were randomly divided into four groups each 10 rats: Groups A as the modeled,B as the treated,C as the positive control,and D as the normal;Groups A,B and C were made into osteoporosis models by whole bilateral ovariectomy but Group D was sham-operated;after the operation,all the groups were routinely bred for 12 weeks and then treated for another 12 weeks: Groups A and D by physiological saline,Group B by oral YGT and Group C by a-D3 solution;after the treatment,all rats were killed and the serum BGP and CT levels were measured by RIA and the osteoblasts of the four groups were observed and compared through HE staining the right proximal tibial pathological sections.The results showed that the serum BGP and CT levels of Group B were significantly higher and the osteoblast numbers were obviously increased compared with the ones of Group A,suggesting that YGT has a function to raise the serum BGP and CT levels,inhibit bone absorption,speed up bone formation and treat osteoporosis.