反相高效液相色谱同时测定动物营养液中脂溶性维生素A、D_3和E

Simultaneous Determination of Fat-soluble Vitamin A,D_(3) and E in Nutrient Solution for Animals by Reversed Phase High Performance Liquid Chromatography

建立了反相高效液相色谱法同时测定动物营养液中脂溶性维生素A、D3和E的方法。采用ZORBAX SB-C18柱（150×2．1mm，5μm），以异丙醇-水（65：35，V／V）为流动相，流速0．3mL／min，在325nm、265nm和290nm三波长下测定。结果表明：该方法的回收率为：VA94．0％～102．0％；VD3 90．9％～99．6％；VE88．6％～92．3％。RSD（n=6）分别为：VA，1．09％；VD3，1．42％；VE，1．23％。检出限为：VA，0．31ng；VD3，0．71ng；VE，0．82ng。

A reversed phase high performance liquid chromatographic method for the simultaneous determination of fat soluble vitamins in nutrient solution for animals was developed. A ZORBAX SB- C18 column（150×2. 1 mm,5μm）was used for separation with isopropanol-water （65： 35） as the mobile phase. The detection wavelengths of vitamin A, D3 and E were at 325 nm, 265 nm and 290 nm, respectively. The results showed that the recoveries and relative standard deviations of vitamin A,D3 and E were 88.6%-102. 0% and 1.09%-1.42%, respectively. The detection limits were 0.31 ng for VA , 0.71 ng for VD3 and 0.82 ng for VE,respcctively.