摘要
采用随机区组和正交试验设计,对蓝靛果芽体组织培养技术进行了系统研究.结果表明:外植体以冬眠枝条经水培后萌发的嫩枝茎段为最好;最佳消毒方法为0.1%HgCl2消毒6min;最佳培养基为MS+6-BA(1.0mg/L)+IBA(0.2mg/L).
Using the experiment design of random and orthogonal tests, technique of tissue culture of Lonicera edulis' shoot were studied. The results are shown: The best explant is the delicate shoot after Winter; The best disinfect method is 0.1% HgCl2, disinfect time is 6 minutes; The best culture medium is MS + 6 - BA (1.0 mg/L) + IBA(0.2 mg/L).
出处
《北华大学学报(自然科学版)》
CAS
2006年第6期549-551,共3页
Journal of Beihua University(Natural Science)
基金
吉林省林业厅科技发展项目(200102)