人干扰素-λ的二级结构及其B细胞抗原表位的预测

Prediction of the Secondary Structures and B Cell Epitopes of Human Interferon-λ

目的：预测人干扰索（IFN）-λ1、IFN-λ2和IFN-λ3的二级结构特征及其B细胞抗原表位。方法：用Ceour-Deleage法预测人IFN-λ1、IFN-λ2和IFN-λ3的二级结构；分别用Hopp-Woods法、Janin法、Zimmerman．Simha法和Bhaskaran-Ponnuswamy法分析各蛋白的亲水性、可及性、极性和柔韧性参数；用Kolaskar-Tongaonkar法预测各蛋白的抗原指数。结合人IFN-λ1、IFN-λ2．和IFN-λ3的二级结构特征。综合评价其B细胞抗原表位。结果：人IFN-λ1、IFN-λ2和WN．IFN-λ2的B细胞识别表位可能分别位于其第47～73和154-166、53～75和159-171、53～77和159～171位氨基酸等区域内或附近，被预测的表位均含有β-转角和无规卷曲结构。人IFN-λ1与IFN-λ2，IFN-λ3的二级结构和含有的B细胞优势抗原表位很相似，而人IFN-λ2和IFN-λ3之间的差别非常小。结论：该预测结果将有助于确定人IFN-λ1、IFN-λ2和IFN-λ3的B细胞表位及其生物学活性部位。

Objective： To predict the secondary structures and B cell epitopes for human interferon（IFN）-λ1, IFN-λ2 and IFN-λ3. Methods： The secondary structures of IFN-λ1, IFN-λ2 and IFN-λ3 were predicted by the method of Geourjon-Deleage, and hydrophility, accessibility, polarity, flexibility were obtained by the methods of Hopp-Woods, Janin, Zimmerman-Simha, Bhaskaran-Ponnuswamy, respectively. Antigenic indexes were predicted by the method of KolaskarTongaonkar. Results： The B cell epitopes of IFN-λ1 IFN-λ2 and IFN-λ3 probably located in or were adjacent to amino acids 47-73 and 154-166, 53-75 and 159-171, 53-77 and 159-171, respectively. All the predicted epitopes contained βtum and random coil structure. IFN-λ1, IFN-λ2 and IFN-λ3 had similar secondary structures and predominant epitopes of the B cells. Conclusion： These findings would be helpful for estimate of the epitopes and activity sites localized within human IFN-λ1, IFN-λ2 and IFN-λ3.