可生物降解高分子超细纤维PLA药物缓释体作用于脑胶质瘤的体外研究

In vitro study of controlled biodegradable high molecular superfine fiber PLA release system for glioma

目的比较可生物降解药物缓释体与单纯抗瘤药物体外抗胶质瘤细胞的作用。方法通过MTT法筛选药物敏感浓度后,应用化学电纺锤工艺将聚乳酸(PLA)与卡氮芥、阿霉素、紫杉醇制成缓释体,并应用高效液相色谱仪测定其缓释率,筛选缓释效果好的缓释体。体外利用L929细胞在空载体浸出液培养后测定吸光度值(OD值),计算相对增殖率(RGR),按六级评分标准评价空载体毒性,0～1级无毒性;利用C6胶质瘤细胞在药物缓释体中培养后计算细胞抑制率(IR)验证药物缓释体的缓释杀瘤效果。结果卡氮芥-PLA缓释体、阿霉素-PLA缓释体缓释平稳,紫杉醇-PLA缓释体缓释效果差并有暴释。空载体毒性为0～1级。卡氮芥-PLA缓释体平稳释放后细胞抑制率为12.3%。结论载体材料无毒性作用,卡氮芥-PLA缓释体缓释效果好,体外杀瘤平稳、持续杀瘤,可进一步通过体内动物实验验证其有效性。

Objective To compare in vitro antitumor effects of controlled biodegradable drug release system and simplex anti-tumor drug in the patients with glioma Methods After selecting optimal dose of drug by MTT method, polylactide （PLA） was mixed with BCNU, adriamycin and taxol, respectively, to make them into controlled release systems by chemical and electric spindle technics, and their controlled release rates were measured by high-performance liquid chromatography （HPLC）, from which the best system was selected based on the release rate, After L929 cells were cultured in empty vector lixivium in vitro, OD value was measured, relative growth rate （RGR） calculated, and toxicity of the empty vector evaluated according to 6-grade scoring criteria, Grades 0-1 are avirulent, Cell inhibition rate （IR） was calculated after C6 glioma cells were cultured in the controlled drug release system for testifying tumor-killing effect of the controlled drug release system. Results Efficacy of controlled release of BCNU-PLA and adriamycin-PLA release systems is steady, but that of taxol-PLA controlled release system is not steady with a sudden release. Empty vector toxicity was at grades 0-1. Inhibition rate of cell growth by controlled BCNU-PLA release system was 12.3%. Conclusion Vector material is not toxic. The controlled BCNU-PLA release system can kill glioma cells in vitro effectively and consistently, and the efficacy can be validated through further animal experiments.