急性白血病患者同源结构域相互作用蛋白激酶2的表达及其临床意义

HIPK2 gene expression and its clinical significance in acute leukemia

目的:探讨急性白血病患者中同源结构域相互作用蛋白激酶2(H IPK2)基因的表达及其临床意义。方法:应用半定量逆转录-聚合酶链反应(RT-PCR)检测急性白血病患者(AL)及健康供者骨髓单个核细胞(MNC)H IPK2基因的表达。结果:①初发AL患者H IPK2的相对转录水平为0.364±0.286,显著低于正常对照组(1.160±0.272)(P<0.01);②AML患者中M2b的H IPK2的转录水平显著高于其它类型的AML(P<0.05);③除M2b外的AL患者H IPK2的转录水平与FAB亚型、发病时外周血白细胞数、细胞或分子遗传学异常、乳酸脱氢酶水平、多药耐药基因1(mdr1)表达、P170表达及年龄等预后因素无显著相关性(P>0.05)。结论:H IPK2在AL患者中低表达,可能与AL的发生发展有关。

AIM： To investigate homeodomain - interacting protein kinase 2 （ HIPK2 ） gene expression level and its clinical significance in acute leukemia （AL） patients. METHODS： HIPK2 mRNA was determined by semi - quantitative reverse transcriptase polymerase chain reaction （semi -quantitive RT- PCR） in patients with acute leukemia and healthy donors. The relative transcription level was compared between the study group and the control group. RESULTS： ① The relative expression level of HIPK2 mRNA in AL patients was 0. 364 ± 0. 286, significantly lower than that in control （1. 160 ±0. 272, P 〈0. 01 ）. ② HIPK2 gene expression level in M2b type was significantly higher than that in other acute myeloid leukemia （AML） subtypes （P 〈 0. 05）.③ HIPK2 gene expression in AL, except M2b, had no relationship with the clinical prognostic factors such as cytogenetic or molecular aberrations, WBC counts, LDH, mdrl, P170 protein and age （ P 〉 0. 05 ）. CONCLUSION ： AL has significantly lower HIPK2 mRNA expression as compared to normal control, which may be associated with leukemogenesis and/or disease progression of AL.