前列腺特异膜抗原剪接变异体的基因结构和多态性分析

Analysis of gene structure and diversity of new alternative spliced variants of prostate specific membrane antigen

目的:了解PSMA剪接变异体的结构及其多样性,探讨前列腺癌的发生机制。方法:应用cDNA末端快速扩增(RACE)的方法扩增剪接变异体5和3末端,并进行序列测定,分析前列腺癌组织PSMA剪接变异体的结构及其多样性。结果:从前列腺癌组织中发现4种新的PSMA剪接变异体,与已往报道的PSMA剪接变异体相比,新发现的变异体在不同位点存在不同程度的插入及缺失。结论:本研究所发现的新PSMA剪接变异体证实和丰富了PSMA剪接变异体的多样性,为寻求前列腺癌特异性的诊断标志物和治疗靶点提供了新的线索和思路。

AIM： To find out the gene structure and diversity of protate specific membrane antigen （PSMA） alternative spliced variants, and probe into the pathogenesis of prostate cancer. METHODS ： 5＇ - RACE and 3＇ - RACE methods were used to amplify the 5＇ and 3＇end of alternative spliced variant and then those viariants were sequenced for analyzing the gene stucture and diversity of PSMA alternative spliced variants of prostate cancer tissues. RESULTS ： Four new alternative spliced variants of PSMA were discovered from prostate cancer tissues. Compared with reported PSMA alternative spliced variants, different insertions and deletions existed in different sites of those new variants. CONCLUSION： The discovery of the new variants confirms the diversity of PSMA spliced variants and provides the clues for seeking the target of diagnosis and therapy of prostate cancer.