骨髓间质干细胞诱导为神经元样细胞移植治疗脑梗塞模型大鼠后运动功能的改善

Transplantation of neuron-like cells induced from rat bone marrow mesenchymal stem cells improves motor function in a MCAO rat model

目的:观察骨髓间质干细胞诱导为神经元样细胞移植对大脑中动脉阻塞模型(MCAO)鼠后运动功能的改善情况。方法:采用分离消化法对F ischer344大鼠的骨髓间质干细胞进行体外培养、纯化鉴定后,进行诱导分化,局部立体定位注射于MCAO大鼠纹状皮质18区,对照组注射无血清L-DMEM基础培养液。分别于诱导细胞移植后第2周及第8周,进行网屏握持试验、抓握牵引试验、平衡木行走试验及Morris水迷宫测试,并取脑组织做2,3,5-三苯四唑化氯(TTC)染色。结果:移植后第2周及第8周对照组、实验组于水迷宫试验中找到目标的时间(s)及路径(cm)均有显著差异(P<0.05);第8周时实验组与对照组相比网屏握持、抓握牵引及平衡木行走等试验的时间(s)亦存在显著差异(P<0.05)。结论:诱导细胞的移植对MCAO模型大鼠的运动功能有明显恢复作用。

AIM ： To observe the amelioration of motor function in a media cerebral artery occlusion （MCAO） rat model after transplantation of neuron - like cells induced from rat bone marrow mesenchymal stem cells. METHODS ： Transplant neuron - like cells derived from rat bone marrow mesenchymal stem cells, which were isolated, cultivated, predicated and induced in vitro, were introduced into infracted cerebral cortex. L - DMEM were injected in control group. Screen test, beam test, prehensile traction test and Morris maze test were conducted at 2 and 8 weeks alternately after transplantation. Rat brain tissues were stained with Trc and percentage of infracted volume was analyzed. RESULTS： There was significant difference between control group and test group in the time and length for rat finding aim and in the grade and time of screen test, beam test, Prehensile traction test （ P 〈 0. 05 ）. CONCLUSION： Transplantation of neuron - like cells induced from rat bone marrow mesenchymal stem cells improves the motor function of MCAO rats.