肾缺血-再灌注损伤引起的脑生化改变

Change of neurobiochemistry induced by renal ischemia-reperfusion injury

目的:观察血清及脑脊液生化成分变化,初步探讨肾缺血-再灌注损伤对脑的影响。方法:20只健康新西兰兔随机分为对照组和肾缺血再灌注组(IR组),检测和比较血清和脑脊液中肌酐(Cr)、尿素氮(UN)、Na+、Ca2+、一氧化氮(NO)代谢产物含量及脑组织总一氧化氮合酶(NOS)活力。结果:在肾缺血再灌注后24 h,IR组血清和脑脊液中的UN、Cr和NO均显著高于对照组(P<0.05),Na+明显低于对照组;IR组血清Ca2+显著低于(P<0.05)、而脑脊液中Ca2+显著高于对照组(P<0.05)。IR组脑组织中NO2-/NO3-含量和NOS活力均明显高于对照组(P<0.05)。结论:肾缺血再灌注损伤不仅影响血清成分,而且还可引起脑生化的改变;NO可能参与了肾缺血-再灌注损伤对脑功能的影响。

AIM： To explore the effects of renal ischemia - reperfusion injury on the changes of biochemistry in blood serum and eerebrospinal fluid. METHODS： Twenty healthy New Zealand rabbits were randomly divided into control group and renal isehemia- reperfusion injury group （IR group）. The contents of ereatinine （Cr）, urea nitrogen （UN） , Na^＋ , Ca^2＋ , metabolite of nitric oxide （NO） in blood serum and cerebrospinal fluid were detected and analyzed. The total activity of nitric oxide synthase （NOS） in brain tissues was also measured. RESULTS： Compared with control group, the contents of UN, Cr and NO were obviously higher （ P 〈 0. 05 ） , and the content of Na ^＋ was obviously lower both in blood serum and eerebrospinal fluid in IR group than those in control at 24 h after renal isehemia - reperfusion injury. The content of Ca^2＋ was lower in blood serum but higher in cerebrospinal fluid in IR group compared with control group （ P 〈 0. 05 ）. Both the contents of NO2^ -/NO3^ - and the total activity of NOS in brain tissues were higher in IR group than those in control group （P 〈 0. 05 ）. CONCLUSION： Not only elements of blood serum but also the biochemistry of cerebrospinal fluid were changed by renal isehemia - reperfusion injury. NO may involve in the influence of the renal isehemia - reperfusion injury on the cerebral function.