小鼠表皮干细胞分化潜能的体内植入研究(英文)

Implantation study on the potential of murine epidermal stem cell differentiation

目的:将小鼠表皮干细胞(epidermal stem cells,ESCs)种植到可生物降解的载体内并移植到同基因受者小鼠皮下,研究其分化潜能。方法:在体外诱导ES细胞分化为ESC,经用Hoechst 33342荧光染色后,种植到含有胶原凝胶的聚乙醇酸(polyglycolic acid,PGA)小网内,作为细胞载体移植到与干细胞同基因的129/J小鼠皮下。结果:荧光显微镜观察发现ESC在移植物内至少能存活3周以上,并能分化形成毛囊样和腺样结构,形成与天然真皮相似的结构。在植入后10周内未观察到明显排斥或毒性反应。结论:ESC可作为种子细胞用于制备具有形成真皮附属结构潜能的皮肤替代物的实验研究。

AIM ： To investigate the potential of murine epidermal stem cell （ ESC ） differentiation after seeded in a biodegradable carrier and implanted subcutaneously into syngeneic recipient mice. METHODS： ES cells were induced in vitro to differentiate into ESCs. After stained with a fluorescent dye Hoechst 33342, these ESCs were seeded into a polyglycolic acid （PGA） net containing collagen gel, functioning as a cell carrier, and implanted subcutaneously into 129/J mice, which were syngeneic to these stem cells. RESULTS： The ESCs kept alive in the implant when observed under a fluorescent microscopy 3 weeks or longer after implantation, and could differentiate into hair follicle - like structure, glandular structure, and gave rise to additional structures displaying features resembling native dermis. No apparent rejection or severe side effects were observed at least 10 weeks post -implantation. CONCLUSION： It is feasible to use these ESCs as seed cells in the study to fabricate dermal equivalent having the potential to develop dermal appendages.