类固醇合成相关酶在小鼠不同组织中的表达

Expression of enzymes associated with steroid biosynthesis in different tissues of mice

利用RT-PCR和Western-blotting技术分析了胆固醇侧链裂解酶(P450scc)、3β-羟甾脱氢酶(3-βHSD)、C17-20裂解酶(P450c17)、StAR mRNA和蛋白在成年昆明小白鼠心脏、脾、肝、肾中的表达。结果显示,StAR mRNA及其蛋白、P450scc mRNA在以上组织中均有表达;3-βHSD在肾中有较强的表达,在其他组织的表达较弱;P450c17在上述组织中无明显的表达,但在睾丸中有明显的表达。这表明,心脏、脾、肝、肾均具有合成类固醇的能力,但合成类固醇的类型存在一定的差异。

mRNA expression levels of cholesterol side chain decomposition enzyme （P450scc）, 3β- hydroxysteroid dehydrogenase（3β-HSD）, cytochrome P450 17 alpha-hydroxylase （P450c17） and steroidogenic acute regulatory protein（StAR） were examined by RT-PCR and the expressed protein levels of StAR were examined by Western-blotting analysis in nonsteroidogenesis tissues including the heart, spleen,liver and kidney of adult Kunming mice. In results, StAR mRNA and protein and P450scc mRNA were expressed in all tissues. 3β-HSD had higher expression level in the kidney than those in the other 3 tissues. P450c17 was expressed in testis of mice. The results demonstrated that the 4 tissues had the ability of steroids biosynthesis, but the types of synthesized steroids were different among these tissues.