小型猪颈动脉粥样硬化性狭窄模型的初步建立

Initial estabilishment of carotid atherosclerotic stenosis model in mini-pig

目的探索建立适合于神经介入研究的动脉粥样硬化性颈动脉狭窄动物模型的方法。方法以12头广西巴马小型猪为实验对象,以高脂饲养和动脉球囊损伤为模型建立的主要手段。应用体外超声、数字减影血管造影(DSA)和血管内超声(IVUS)评价颈动脉狭窄的形成情况,并对颈动脉的病理改变特点进行评价,最后分析总结出创建动脉粥样硬化性颈动脉狭窄动物模型的理想方案。结果16周时的DSA发现：高脂饲养和动脉球囊损伤组平均狭窄率达33．32%±12．84%；病理检查证实有明显的粥样硬化斑块形成。结论高脂饲养加动脉球囊损伤,16周可以初步建立广西巴马小型猪颈动脉粥样硬化性狭窄模型。

Objective To explore a protocol for establishing an animal model suitable for experimental studies of the intervention of carotid atherosclerotic stenosis （CASS）. Methods Twelve Guangxi BA-MA Mini-Pigs were ehosen. High fat/cholesterol feeding and endovascular balloon denudation are the two main methods in this study. Duplex ultrasound, digital subtraction angiography （DSA） and intravaseular ultrasound （1VUS） were used to find and evaluate the carotid stenosis and the atheroselerotic plaque, Once satisfactory stenosis and atherosclerotie plaque were found, artery, specimens would be gained after intravital peffusion and fixation with 4% neutral formalin. Histological methods, such as hematoxylineosin staining （HE）, Van Gieson staining （VG）, Masson staining and several immunohistoehemieal analysis were used to investigate the characteristics of the atheroselerotic lesions, and then to screen the best protocol for developing an ideal CASS animal model. Results At the 16th week, DSA demonstrated the average rate of stenosis of the high fat/cholesterol feeding and injury group （HF＆I） was 33. 32% ± 12. 84%, And the pathological studies demonstrated that evident atherosclerotic＂ plaques had formed on these arteries, Conclusions A feasible animal model of carotid atheroselerotic stenosis can be established in Guangxi BA-MA Mini-Pig by the means of balloon injury and high fat/cholesterol feeding for about 16 weeks as described by the authors.