顶空气质联用法测定腐败血、肝检材中CO

Microanalysis of carbon monoxide in blood and hepatic tissues by head-space capillary gas chromatography and mess spectrum

目的研究CO中毒腐败血、肝组织检材中CO的HS/GC/MS检测。方法用HS/GC/MS法分析碳氧血红蛋白(COHb)血的线性范围。配制10%、30%、50%、70%浓度COHb血样,分别在室温、冷藏、冷冻条件下保存,分别在当日、第4、14、45d进行测定,比较实验结果。腐败肝组织由雄性健康家兔通CO气体致死,当天解剖,家兔肝常温隔绝空气保存并放35d至腐败,期间进行不定期顶空测定分析。结果制备的COHb血在0-100%之间有良好的线性关系Y=2.4X+2.2(r=0.9995)。以此方法测定家兔CO中毒致死的COHb新鲜血的浓度和4℃下放置45dCOHb腐败血,结果表明温度对血样中COHb%的测定影响最大。采用HS/GC/MS法检测,每次只需0.25ml血样或1g肝脏,分析一次时间只需3min,均可检测出新鲜检材和常温放置45d的腐败肝组织检材CO的含量。结论HS/GC/MS法能检出CO中毒的腐败生物检材中CO。

Objective To introduce a method for the determination of carboxy-haemoglobin （COHb） in the putrid blood and hepatic tissues. Methods The standard curve of CO and COHb% was determined by the method of HS/GC/MS. The blood samples had been preserved for 4 days, 14 days and 45 days respectively. Putrid hepatic tissues came from male rabbits which died of carbon monoxide and preserved for 35 days out of air at normal temperature and in the meanwhile the CO was analyzed for several times. Results The standard curve equation of COHb% （0-100% ） was Y = 2.4X ＋ 2.2 （r = 0. 9995 ）. The temperature had heavily effect on the COHb level of blood samples. It needs only 0.25ml blood samples or 1g hepatic tissues for 3mins each time by this method. The CO content could be determined in both fresh and putrid hepatic samples which have been preserved in tubes at normal temperature （ 15 ～ 17℃ ） for 45 days. Conclusion The methods reported in this paper are proper for the CO determination of hepatic biological samples in the case of carbon monoxide poisoning.