依达拉奉对脑外伤后大鼠的神经保护作用

Neuroprotective effect of edaravone on cerebral trauma rats

目的研究自由基清除剂依达拉奉对大鼠颅脑外伤的保护作用。方法将SD大鼠分为依达拉奉组(n=32)和对照组(n=32)。利用自制自由落体撞击装置,制作脑外伤模型。撞击前30min及伤后3h尾静脉给药。分4个时间点于外伤后6h、24h、3d、7d分别处死实验组和对照组。TUNEL法检测凋亡,免疫组化法分析8-羟基-脱氧鸟苷。结果外伤后初期依达拉奉对大鼠生理指标没有明显的影响(P>0.05);但依达拉奉治疗后的脑外伤大鼠相对对照组神经功能评分明显减少(P<0.05);神经元的凋亡情况也有显著的减少(P<0.05),同时显著减少外伤后脑组织中8-羟基-脱氧鸟苷含量(P<0.05)。结论依达拉奉对脑外伤大鼠有很好的神经保护作用,其作用机制可能与清除氧自由基,减轻脑水肿有关。

Objective To investigate the ncuroprotective effect of edaravone, a kind of free radical scavenger on cerebral trauma rats. Methods To separate SD rats into group experimental （n=32） and group control （n=32）. Knock-on device was used to create cerebral trauma model. Both groups were administered with edaravone by caudal vein 30 minutes before and 3 hours after knocking. To execute the two groups of rats after injured 6 hours, 24 hours, 3 days and 7 days, detected cerebral cell apoptosis by TUNEL, and analyzing 8-hydroxy-deoxyguanosine levels by immunohistochemistry. Results Edaravone seemed to put no significant influence on physiological index of rats during the early period of injury （P 〉0.05 ）. But it obviously reduced neurologic function score of group experimental compared to group control （P 〈 0.05 ）. Meanwhile, the neuron apoptosis of group experimental significantly decreased as well. Edaravone markedly suppressed the 8-hydroxy-deoxyguanosine in injury tissue （P 〈0.05 ）. Conclusion Edaravone exerted neuroprotective effect on cerebral trauma rats. And the mechanism is possibly related to scavenging the free radicals and reducing brain edema.